Materials and Methods

Syrian hamsters (6-8 weeks old) were divided into two groups. The control group (n = 6) received SC implants of 25 mg cholesterol pellets, and the treated group (n = 19) DES pellets every 3.0 mo (2, 3) in combination with ethylnitrosorea (ENU)

(3). For screening the mutagenic potential of Es, the mouse Leydig TM3 cells (ATCC) were treated with 100 ng/ml of 17a- and (3-E2, DES, bisphenol A, a-zearalanol or the vehicle (ethanol). After 72 hs of treatment, the cells were collected and genomic DNA was extracted. Sets of twenty OPA, OPC, OPK, OPE, OPAA and OP-26 oligonucleotide random 10-mer primers were from Operon Technologies, Alameda, CA. RAPD-PCR was performed as previously described by us (4).

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