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A typically between 100-300 families were analyzed per AB-QTL population b * indicates populations phenotyped in a single environment minimum of 3 for total red yield to a maximum of 88 for soluble solids content. Over ten traits common to all five studies, the highest percentage of positive QTL was identified in the S. pimpinellifolium study (44 ), followed by the S. peruvianum (41 ), S. neorickii (28 ), S. pennellii (27 ) and S. habrochaites (15 ) studies (Grandillo and Tanksley 2005)....

Other Tilling Services

In addition to the services mentioned above, there are several TILLING services currently being offered by groups not affiliated with the STP. The Lotus TILLING facility (Perry et al 2003) began accepting screening orders in June of 2003 with a population of 5000 M2 plants along with smaller populations enriched for defects in symbiosis and in starch accumulation. The Lotus facility has switched from the ABI377 to the Li-Cor DNA Analyzer and has reported the discovery of over 100 EMS induced...

Model Plant Species For The Genomicsbased Studies

Nearly twenty years ago, plant biologists were looking for a model organism suitable for detailed analysis using the combined tools of genetics and molecular biology. Plants with effective protocols for regeneration in culture (such as Petunia and tomato) were logical candidates, particularly for studies involving Agrobacterium- mediated cell transformation. However, attention gradually shifted towards Arabidopsis, a small weed in the mustard family that was first chosen as a model genetic...

Comparative Genomics As A Tool For Gene Discovery And Marker Development

Comparative genomic studies have increased knowledge about the level of conservation between the cereal genomes and led to the generation of genomic tools that can be used to define efficient strategies for genetic studies and gene isolation in these genomes. 5.1. Colinearity-Based Gene Cloning in Cereals In some cases, the conservation of sequence at orthologous positions between the genomes can reflect the conservation of a gene with a similar function between species. Early comparative...