Expression Vector and Identification of an Expression Strain

Polymerase chain reaction (PCR) techniques were used to identify two alcohol oxidase-encoding genes in P. methanolica AUG1 and AUG2 (alcohol utilization gene Raymond et al, 1998). The alcohol oxidases encoded by these genes share 83 identity with one another. Auglp shares 84 identity with Moxlp from Hansenula poly-morpha, 72 identity with Aodlp from Candida boidini, and 69 identity with Aoxlp from P. pastoris. Three lines of evidence indicate that Auglp is the dominant alcohol oxidase in P....

Advantages and Disadvantages of Adenoviral Vectors

The unique advantage of the adenoviral expression system is its versatility. Once constructed, a single vector can be used for in vitro protein expression and purification, studies of the effect of the gene product on cell biology, or in vivo studies in many tissue types and a number of different species. For these reasons, adenovirus is becoming the system of choice for work involving both in vitro and in vivo studies. The investigator has the option of using either well-characterized viral...