Cr1 Cr2

IGF-1 1 (ng/ml)

094 ± 11

9 864 ± 79b

773 ± 60b'

c 627 ± 90a 346 ± 40b n/a

n/a 266 ± 23a 1

70 ± 11 b n/a

Insulin (ng/ml)

102 ± 49

15 ± 10b

18 ± 10b

22 ± 18a 20 ± 26

28 ± 30 21 ± 19a

n/a m/a

Glucose (mg/dL)

126 ± 34

112 ± 12

102 ± 4

106 ± 18 90 ± 16

91 ± 12 106 ± 17

n/a n/a

Data represent means ± SD (n = 3 to 6). ap < 0.05 vs (—/—)-AL group, bp < 0.05 vs the group AL in each rat group, cp < 0.05 vs the group CR1 in each rat group by a post hoc test (Fisher's protected least significant difference test) for multiple comparisons after 1-factor ANOVA. AL, groups of rats fed ad libitum. CR1 and CR2, groups of rats subjected to 30% calorie restriction, while sacrificed in the first day and the second day of 2-day feeding cycle, respectively (see the rat husbandry for the CR regimen, a modified alternate-day-feeding, used in the present study).

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