Dietary Restriction and Oxidative Stress in Annual Fish Mutants

Once the mutagenesis is achieved, dietary resistant mutants and oxidative stress resistant mutants could be selected which might suggest genes involved in such a mechanism. For example a paraquat resistance mutant could be selected and then tested for lifespan extension. Similarly, mutants could be fed with a high fat and high nutrient diet which, in contrast to dietary restriction, should shorten the lifespan and the mutants that live under these conditions could be selected. However, these require careful feeding experiments. Likewise, by feeding !-3 fatty acids, the mutants that do not prolong the lifespan in the presence of !-3 fatty acids could be selected. All these imaginative screens will be feasible once the Nothobranchius model is available as a genetic model.

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