HDV Is a Poor Immunogenic Protein

In several studies immunization of mice or woodchucks with DNA vaccines failed to induce a detectable humoral immune response. In the study of Mauch et al. none of more than 120 mice of different haplotypes that were immunized with plasmids expressing either S- or L-HDAg developed a detectable anti-HDAg response. Failure to detect anti-HDAg was not due to lack of HDV specific T-cell help, as a CD4+ Th-cell immune response was induced by all plasmids. Seizer et al. demonstrated that immunization...

Hepatitis Delta Virus

Department of Microbiology and Immunology The cover illustration is a simplified structure of hepatitis delta virus showing the internal ribo-nucleoprotein complex, which contains the circular RNA genome and the two forms of the hepatitis delta antigen the envelope proteins ofhepatitis B virus form the exterior of the virus. The inset is an electron micrograph of purified hepatitis delta virus particles, and was kindly provided by Dr. John Gerin. The background immunofluorescence image is...

Immunization with HDV Protein

Prokaryotic and eukaryotic expression systems were used to produce HDV protein for vaccination. Different expression systems result in different protein folding and, therefore, different presentation to the immune system. Karayiannis et al. studied the immunogenicity of HDAg expressed in E. coli in 1990 (Karayiannis et al. 1990). They immunized four WHV carrier wood-chucks with a fusion protein consisting of the 64 N-terminal amino acids of HDAg and a part of the MS2 polymerase of the bacterial...