Sesep E P62

Because the conversion of ES to EP does not involve binding or release steps, its speed is independent of the concentration of reactants. Thus we can further simplify:

If the conversion of ES to E + P is much slower than the binding of S to E (rapid equilibrium assumption), the reaction velocity (rate of product formation or substrate consumption) will be proportional to the concentration of ES:

By the law of mass action, the concentration of ES depends on the concentration of E and S:

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