Problems of Chapter

a) True, the STOKES-radius depends on both size and shape of proteins and determines whether or not they fit into the pores of the gel.

b) True, separation is probably by a mixture of ion exchange and affinity chromatography.

c) False, SDS-PAGE separates by molecular weight. Binding of SDS gives proteins a high negative charge, irrespective of their pI.

d) True, substrate binding reduces conformational freedom and thus stabilises the protein against denaturation.

e) True, as this would result in protein inactivation.

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