DNA Sequence Analysis ofImmunodominant Proteins

The detected N-terminal amino acid sequence of immunodominant proteins were compared with known amino acid sequences of various protein data banks and with data of unfinished Staphylococcus aureus genome sequencing. Large contigs, which include sequences that matched to 100 % with the predicted N-terminal amino acid sequence of immunodominant proteins were analyzed for open reading frames (ORFs). ORFs, where the presumed molecular weight of the protein product was similar to the molecular weight of immunodominant protein determined by immunoblotting, were cloned in Escherichia coli and subsequently sequenced.

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