Utility Of The System

The clinical application of autologous chondrocyte implantation to the treatment of articular cartilage defects has stimulated the development of highly reproducible, standardized methods for human articular chondrocyte isolation and culture. The autologous nature of the chondrocyte implantation procedure requires that the cell culture medium must promote rapid proliferation and maintenance of re-differentiation potential of adult human articular chondrocytes in patients of varied health and...

Cardiac Protein Gene Expression

Characterization of cardiac-specific gene expression permits confirmation of Western blotting or immunohistochemical staining evaluation of protein levels, as well as allowing evaluation of cellular differentiation and time-dependent de-differentiation in culture. Specific gene expression can be quantified by Northern blot analysis. As the level of contractile proteins within the cultured cells decreases with time, RT-PCR may be employed in order to permit amplification of increasingly weak...

Development of a defined medium for articular chondrocyte culture

Human articular chondrocytes were initially seeded at 3-4000 cells per cm2 and allowed to attach to tissue culture plastic for one day in DMEM supplemented with 10 FBS. Following overnight incubation, culture medium was removed and replaced with DMEM FBS as control, or the defined medium, as described below. Figure 2. Comparison of chondrocyte culture in autologous human serum versus fetal bovine serum. Human articular chondrocytes were cultured in medium supplemented with fetal bovine serum or...