The Vp1 Pentamers Can Be Transported To The Nucleus With Vp2 Or Vp3

How are the capsid proteins transported to the nucleus It has been thought that VP1 is associated with VP2 or VP3 in the cytoplasm and transported to the nucleus cooperatively. JCV VP1 was distributed in both the cytoplasm and the nucleus in the absence of VP2 and VP3, but predominantly localized to the nucleus in their presence (Fig. 8.8B-D). The NLS-defective SV40 VP1 and murine polyoma virus VP1 were distributed in the cytoplasm, but localized to the nucleus in the presence of wild-type VP2...

References

Gardner SD, Field AM, Coleman DV, Hulme B (1971) New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet i 1253-1257. Padgett BL, Walker DL, Zu Rhein GM, Eckroade RJ, Dessel BH (1971) Cultivation of papova-like virus from human brain with progressive multifocal leucoencepha-lopathy. Lancet i 1257-1260. Spencer ES, Andersen HK (1970) Clinically evident, non-terminal infections with her-pesviruses and the wart virus in immunosuppressed renal allograft recipients. BMJ...

Introduction

Following the 1965 demonstration by Gabriele Zu Rhein and Shi-Ming Chou (Zu Rhein and Chou, 1965) and independently by Lloyd Silverman and Lucien Rubenstein that oligodendrocytes in the brain lesions of patients with progressive multifocal leukoencephalopathy (PML) contained numerous particles that looked like papovavirus virions, it was inevitable that many virologists would try to isolate and cultivate the virus in cell cultures or experimental animals. In electron micrographs the particles...

Widening Our Collaboration

We thought that broadening our proposal might improve our chances, so we turned to colleagues in the Department of Veterinary Science. The Departments of Medical Microbiology and Pathology in the Medical School and the Department of Veterinary Science in the College of Agriculture (this was before the University had a School of Veterinary Medicine) had for many years main tained a cordial working relationship in both research and graduate teaching. Within the Department of Veterinary Science...

Info

Sam Chou and myself with our poster exhibit ''Polyoma-like Virions in a Human Demyelinating Disease'' at the annual meeting of the American Academy of Neurology, in Philadelphia, in 1966. Figure 2.3. Dr. Sam Chou and myself with our poster exhibit ''Polyoma-like Virions in a Human Demyelinating Disease'' at the annual meeting of the American Academy of Neurology, in Philadelphia, in 1966. The first European recognition came from Dr. W. Bernhard, Director of the Cancer Research...

Jcve666s

AMutants are described by Mandl et al. (1987 mutants 1-8), Lynch and Frisque (1991 mutant 9), Trowbridge and Frisque (1995 mutant 10), Kelley and Georgopoulus (1997 mutant 11), Tavis et al. (1994 mutants 12 and 13), Tavis and Frisque (1991 mutants 14-23), and Swenson et al. (1996 mutants 24-31). Mutations alter one or more of the following JCV early proteins TAg (T), tAg (t), all three T' proteins (T'), or a single T' protein (T165). Introducing insertions or deletions at convenient restriction...

Human Cancer

Until recently, evidence for the involvement of JCV, BKV, and SV40 in human cancer was virtually nonexistent. The advent of polymerase chain reaction (PCR) technology, however, has sparked renewed interest in the issue and provided provocative evidence that these viruses may indeed play a role in certain neoplasms. The fact that these viruses can indeed cause tumors is not disputed, based on the in vitro studies described above as well as experiments in animals. For example, SV40 induces...

The Comingout Party

On December 5, 1964, with several illustrious virologists presiding on a dais and Dr. Chou watching intently from the balcony, I presented our two cases, followed by Dr. Rubinstein. His case had never been in formalin. At once Dr. Sabin took over and tore into us with vigor. ''This is deplorable, everybody thinks everything is a virus'' (quote from my diary), and ''One cannot say one has morphologic evidence of a virus,'' and, with regard to the electron micrographs, ''That is a good way not to...

The Start Of A Collaboration

Gabriele Zu Rhein clearly wanted to see this virus cultivated and characterized. She had demonstrated its presence in oligodendrocytes in PML brain tissue. The logical next step was to cultivate and identify it in order to study its role in nervous system disease. She wanted to have a part in that process, but she did not have the virology laboratory or virology background to do this, so she began prodding me to take it on in a collaborative project. I had a virology laboratory in the...

Arrival Of Pml Cases And An Electron Microscope

Another interdisciplinary contact, which became of considerable importance later on, developed with Veterinary Science. In this Department, Dr. Carl Olson had created a research unit for the study of papilloma viruses, especially of bovine and canine types. A viral oncologist who appreciated the contributions of morphology, he early had acquired an electron microscope. My affiliation with Veterinary Science began in 1957. All such students had to do course work in pathology, and I became their...