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Alphavirus

Colon carcinoma

(143)

mRNAs encoding N, P, M, G, and L (27,28). The five individual mRNAs are capped and polyadenylated. The polymerase recognizes viral nucleotide sequences at the 3'-end of the genome and initiates transcription. Termination probably occurs after polyadenylation whereupon the polymerase is freed to reinitiate transcription of the next gene product (26). The mechanism that allows the polymerase to switch from transcription to replication has not been entirely clarified, although following mRNA and viral protein synthesis, negative-sense progeny genomes are generated in high quantities. The newly synthesized N, P, and L proteins associate in the cytoplasm and form RNP cores, which bind to regions of the plasma membrane now rich in both M and G proteins. VSV particles form and budding/release of progeny virus occurs followed by cellular lysis (26,35).

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