All species are essentially arboreal and use nests of some kind. Mouse lemurs (Microcebus and Mirza) construct globular leaf-nests but can also use tree hollows, whereas dwarf lemurs (Cheirogaleus), hairy-eared mouse lemurs, and fork-crowned lemurs typically use tree hollows.

Dwarf and mouse lemurs are found in every kind of forested habitat in Madagascar, including evergreen rainforest in the east, deciduous forest in the northwest and west, and semiarid forest in the southwest and south. Mouse lemurs (Microcebus species) also occur in small patches of scrub vegetation and even in gardens in settled areas.

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