Cellulases from H insolens

The other commercially interesting fungus, H. insolens, produces an almost identical pattern of cellulases to T. reesei, having at least five EGs, two CBHs, and several ^-glucosidases. These enzymes are also structurally similar, thus belonging to same glycosyl hydrolase families (Table 3). In addition, there exists remarkable homology (> 50%) in the amino acid sequences between the corresponding cellulase component of T. reesei and H. insolens. However, several H. isolens cel-lulases work at higher pH and higher temperatures than T. reesei enzymes and have therefore found commercial applications, especially in textile treatments

Two H. insolens cellulases, EG I and EG III, are lacking the CBD. EGs I-III show similar activity toward CMC and amorphous cellulose, while EGs V-VI have the highest catalytic activity on amorphous cellulose. None of them has been reported to be active in hydrolyzing xylan. H. insolens CBH I degrades crystalline cellulose, but it is clearly less sensitive to end product inhibition than CBH I from T. reesei.

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