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continued

Table 9 Continued

Phytase from

Original strain or overexpressed in

Method

Remarks

Reference

M. thermophila

P. involtus Phy 1

P. involtus Phy 2

S. cerevisaea

A. niger NW205 or H. polymorpha

A. cryzae IFO4177

A. oryzae IFO4177

1. Hydrophobic interaction chromatography Butyl Sepharose Fast Flow

2. Gel permeation chromatography Sephacryl S-300

1. Desalting Fast-Desaltin HR 10/10 or Sephadex G-25 superfine

2. Anion exchange chromatography Poros HQ/M

1. Anion exchange chromatography Q-Sepharose Fast Flow

2. Hydrophobic interaction chromatography Butyl Toyopearl 650S

3. Desalting Sephadex G25

4. Anion exchange chromatography Q- Sepharose Fast Flow

5. Desalting Sephadex G25

6. Anion exchange chromatography Source 30Q

7. Hydrophobic interaction chromatography Phenyl Toyopearl 650S

1. Hydrophobic interaction chromatography Phenyl Sepharose Fast Flow

2. Hydrophobic interaction chromatography Butyl Toyopearl 650S

3. Desalting Sephadex G25

4. Anion exchange chromatography Q-Sepharose Fast Flow

5. Anion exchange chromatography Source 30Q

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