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Figure 6 Sephadex G-75 gel filtration of a crude extract of rat liver. The column (2.5 x 75 cm) was equilibrated at 4°C with 0.05 M sodium acetate buffer, pH 5.0, and eluted with the same buffer. The flow rate was 1 mL/min. Fractions of 10 mL were collected. Ribonuclease activity was expressed as the reading of the absorbance at 260 nm (see Sec. V). Fractions indicated by a bar were pooled.

Figure 6 Sephadex G-75 gel filtration of a crude extract of rat liver. The column (2.5 x 75 cm) was equilibrated at 4°C with 0.05 M sodium acetate buffer, pH 5.0, and eluted with the same buffer. The flow rate was 1 mL/min. Fractions of 10 mL were collected. Ribonuclease activity was expressed as the reading of the absorbance at 260 nm (see Sec. V). Fractions indicated by a bar were pooled.

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