Presence of Irreversible Inhibitor in Reaction System

Examples of irreversible inhibitors are Pb , Hg and Ag+ ions that might be in the water used. Sulfhydryl enzymes (where the sulfhydryl group of cysteine is essential for enzyme activity), which are 25-30% of all enzymes, would be affected. Experimentally, no enzymatic activity would be found until sufficient enzyme is added to bind all the inhibitor. Then, enzyme activity will be seen. This case is easily recognized as the intercept on the x-axis of the vo vs. [E]o plot would be on the right side of the zero intercept.

action effect, and vo will increase. Such effects were observed as early as the 1940s and were called "dilution effects,'' meaning as one diluted the enzyme concentration the activity observed was less than that expected.

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