Complementation Analysis How Many Genes are Involved

Complementation analysis is used to determine whether two independent mutations are alterations in the same gene that is, they are alleles, or are alterations in different genes. In essence, a complementation analysis is a functional test used to define a gene. If a researcher has isolated a number of mutants with a similar phenotype, the next question asked is 'How many genes have I identified '. If there are 10 mutant strains, are they each in different genes, does each mutant carry a...

Classes Of Saccharomyces Cloning Plasmid Vectors

Saccharomyces plasmids were developed from Escherichia coli plasmid vectors. The basic E. coli vector is small 2-4 kilobasepairs (kbp) of DNA and includes genes needed for plasmid replication, an origin of replication (ORI) derived from an E. coli plasmid, and a selectable marker gene such as AMPr (for ampicillin resistance) to be used to identify E. coli transformants containing the plasmid. The E. coli ORI allows the plasmid to replicate independent of the E. coli chromosome as an...

Saccharomyces Genome And Nomenclature

Saccharomyces cerevisiae has a haploid chromosome number of 16. The entire Saccharomyces genome of strain S288C is sequenced and available on the Saccharomyces Genome Database (called SGD) at http genome-www.stanford.edu Saccharomyces. The site has a variety of tools for sequence analysis that are particularly useful for the Saccharomyces researcher, including gene and restriction maps of the chromosomes. The site is interconnected with genome databases for other genetic model organisms and...