Experimental Section A Pectin Glycerol Ester

HM-pectin (1 g, 73 DE, GENU) was partially swollen in 20 mL of glycerol. Then 50 mg of lipase from Candida cylindreacea (Lipase OF-360, Meito-Sangyo) was added to the solution and the mixture was stirred a 55 C for 24 h. The modified pectin was precipitated in methanol and recovered by filtration. After being washed extensively with methanol, the product was obtained as a powder. The yield was 0.98 g. Analysis of the product (MW 125,000, MW Mn 6.8) by size-exclusion chromatography indicated...

Pectin Glycerol Ester

Pectin is extracted from citrus peel, apple pomace, sugar beet, and other plant materials. Structurally it comprises polygalacturonic acid and its methyl ester, and a number of neutral sugars 15 . It is widely used in food applications (e.g., jams and jellies, confectionery, beverages, desserts) and in some pharmaceutical applications. It is susceptible to enzyme reactions, and the following three pectolytic enzymes are well known polygalacturonase (cleaving the galacturonan backbone), pectin...

Strategy 1 Synthesis of Maidong Saponin C

Maidong, one of the most commonly used Chinese herbs, is held to nourish ''yin'' (tonic effect yin is one of the basic philosophical concepts in traditional Chinese medicine). Saponins are believed to be the active principles in Maidong plants, which are found in several species, including some in the Ophiopogon and Liriope genera. Thus far more than 60 steroidal saponins have been isolated from these species 2c . Yu et al. isolated Maidong saponin C (16) from the tuber of Liriope muscari 12 ....

C 013 and 14Galactosyltransferases

Gallon Phosphate Buffer Solution

Though not widely available yet, the 1,3-galactosyltransferase that synthesizes Gal 1 3GlcNAc type I from GlcNAc acceptors has been cloned and expressed 77,78 . Mapping has indicated that N-acyl analogs can be utilized as acceptors by Figure 5 Unnatural donors and acceptors for a ,3-galactosyltransferase. Figure 5 Unnatural donors and acceptors for a ,3-galactosyltransferase. the enzyme 79 . a1,4-Galactosyltransferase from Neisseria meningitidis transfers Gal from UDP-Gal donors solely to...