Experimental Section A Pectin Glycerol Ester

HM-pectin (1 g, 73% DE, GENU) was partially swollen in 20 mL of glycerol. Then 50 mg of lipase from Candida cylindreacea (Lipase OF-360, Meito-Sangyo) was added to the solution and the mixture was stirred a 55°C for 24 h. The modified pectin was precipitated in methanol and recovered by filtration. After being washed extensively with methanol, the product was obtained as a powder. The yield was 0.98 g. Analysis of the product (MW = 125,000, MW/Mn = 6.8) by size-exclusion chromatography indicated around 2-3% increase in the average molecular weight from the starting material (MW = 123,000, MW/Mn = 6.4), corresponding roughly to a degree of esterification of 2% (with glycerol). 1H NMR analysis showed the presence of glycerol moiety. The aqueous solution viscosity of the modified product slightly increased in comparison to the starting pectin.

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