HM-pectin (1 g, 73% DE, GENU) was partially swollen in 20 mL of glycerol. Then 50 mg of lipase from Candida cylindreacea (Lipase OF-360, Meito-Sangyo) was added to the solution and the mixture was stirred a 55°C for 24 h. The modified pectin was precipitated in methanol and recovered by filtration. After being washed extensively with methanol, the product was obtained as a powder. The yield was 0.98 g. Analysis of the product (MW = 125,000, MW/Mn = 6.8) by size-exclusion chromatography indicated around 2-3% increase in the average molecular weight from the starting material (MW = 123,000, MW/Mn = 6.4), corresponding roughly to a degree of esterification of 2% (with glycerol). 1H NMR analysis showed the presence of glycerol moiety. The aqueous solution viscosity of the modified product slightly increased in comparison to the starting pectin.
Was this article helpful?
Now if this is what you want, you’ve made a great decision to get and read this book. “How To Cure Yeast Infection” is a practical book that will open your eyes to the facts about yeast infection and educate you on how you can calmly test (diagnose) and treat yeast infection at home.