This reaction has also been observed in Pseudomonas [A2697], Sporotrichum [B124], Trichosporon [B368, D232], Arthrobacter [H270], Aspergillus [H236], Bradyrhizobium [J484] and Streptomyces [H347].
Burkholderia enzyme is apparently a dimmer; the molecular weight was found to be 67 000 or 78 000 by different methods. It contains two Fe/mol and is stimulated by Fe2+ and ascorbate. The product is 2,4-dihydroxy-5-methyl-cis, cis -muconic semialdehyde. Other catechols are also substrates [J903].
3-Methylcatechol 0 2-methyl-cis, cis-muconate
This reaction has been observed with protocatechuate 3,4-dioxygenase (E.C. 22.214.171.124) from Hydrogenophaga palleroni [J218], catechol 1,2-dioxygenases (E.C. 126.96.36.199) from Pseudomonas, Alcaligenes eutrophus, Trichosporon cutaneum, Rhodococcus rhodochrous and R. erythropolis, as well as by activated sludge microorganisms [A3769, B754, C84, D247, E31, J703].
4-Methylcatechol 0 2-hydroxy-5-methyl-cis, cis-muconic semialdehyde
Bacillus stearothermophilus catalyses this reaction [A2397], and many reports suggest that this reaction may be common in other microorganisms.
4-Methylcatechol 0 3-methyl-cis, cis-muconate
This reaction has been observed with protocatechuate 3,4-dioxygenase from Hydrogenophaga palleronii [J218], and catechol 1,2-dioxygenases from Pseudomonas, Alcaligenes eutrophus, Trichosporon cutaneum, Rhizobium trifolii, Aspergillus niger, Candida tropicalis, Rhodococcus rhodochrous and R. erythropolis [A3769, B754, C84, C342, D247, D672, E204, G647, J703].
4-Methylcatechol 0 2-hydroxy-4-methyl-cis, cis-muconic semialdehyde
Candida tropicalis catalyzes this reaction [A943], and many reports suggest that this reaction may be common in microorganisms.
3-Methylcatechol 0 2-hydroxy-6-methyl-cis, cis-muconic semialdehyde
This reaction has been observed in several subspecies of Pseudomonas putida, in Burkholderia cepacia, Sphingomonas and Bacillus stearothermophilus, in activated sludge microorganisms [A2397, E31, J654, K5], as well as being catalysed by chlorocatechol 2,3-dioxygenase and 1,2-dihydroxynaphthalene oxygenase (see below).
Pseudomonas enzyme is an octamer, monomeric molecular weight 33 200. The product is 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate. Catechol, 3- and 4-methylcatechol are poor substrates [G771]. P. pseudoalcaligenes and P. aeruginosa enzymes, molecular weights 250 000 and monomeric molecular weights 31 000, contain Fe2 + . They are specific for the 1,2-position of 2,3-dihydroxybiphenyl [E329].
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The term vaginitis is one that is applied to any inflammation or infection of the vagina, and there are many different conditions that are categorized together under this ‘broad’ heading, including bacterial vaginosis, trichomoniasis and non-infectious vaginitis.